Western blot analysis of Rat Kidney showing detection of ~95 kDa Axin-1 protein using Rabbit Anti-Axin-1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-1 Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~95 kDa.
AXIN1 / Axin-1 Antibody (Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Rat |
| Format: | Liquid |
$485.00each
Catalog Number: LS-C775204-100
Catalog Number: LS-C775204
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- Description
- Axin-1 antibody LS-C775204 is a biotin-conjugated rabbit polyclonal antibody to Axin-1 (AXIN1) from human. It is reactive with human and rat. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AXIN1 | Axis inhibitor 1 | AXIN | Axin 1 | Axis inhibition protein 1 | Fused, mouse, homolog of | HAxin | PPP1R49 | Axin-1
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O15169
- NCBI GenBank
- AAC51624.1 | AF009674
- SwissProt
- O15169
Target
- Target
- Human AXIN1 / Axin-1
- Antigen Species
- Human
- Epitope
- Detects 95 kDa.
- Gene Name
- AXIN1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the mid-protein of Human Axin-1 (aa. 470-570)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS