Western analysis of AURKA / Aurora-A antibody. Blot of HeLa cells treated with 100 ng/mL of nocodazole for 18 hours was probed with AURKA / Aurora-A antibody. Nocodazole is a microtubule inhibitor which induces cells G2/M phase and induces Aurora A expression. The AURKA / Aurora-A antibody monoclonal binds strongly and cleanly to a band at about 46 kDa.
AURKA / Aurora-A Antibody (clone 3H1)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$384.00each
Catalog Number: LS-C204509-100
Catalog Number: LS-C204509
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- Description
- Aurora-A antibody LS-C204509 is an unconjugated mouse monoclonal antibody to human Aurora-A (AURKA). Validated for ICC, IF and WB.
- Application
- ICC, IF, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AIK | AIRK1 | ARK1 | Aurora-A kinase | AURKA | Aurora kinase A | Aurora/IPL1-like kinase | AYK1 | AURA | AURORA2 | Breast tumor-amplified kinase | Breast-tumor-amplified kinase | BTAK | HARK1 | IPL1-related kinase | Serine/threonine kinase 6 | STK7 | STK6 | PPP1R47 | ARK-1 | Aurora 2 | Aurora-A | Aurora-related kinase 1 | Aurora/IPL1-related kinase 1 | IAK1 | Serine/threonine kinase 15 | STK15
- Recommended Dilution: IF/ICC
- 1:500 - 1:1000|0.388888888888889 - 1:1000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O14965
- NCBI GenBank
- NM_003600 | NP_003591.2
- SwissProt
- O14965
Target
- Target
- Human AURKA / Aurora-A
- Antigen Species
- Human
- Epitope
- Clone 3H1 recognizes Aurora A kinase specifically both in western blots and in immunocytochemical experiments. On blots, clone 3H1 reveals a prominent 46 kDa band, on cells in tissure cultures, clone 3H1 reveals strong staining in spindle poles and midbodies.
- Gene Name
- AURKA
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 3H1
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Full length recombinant human Aurora A expressed in and purified from E. coli.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 10 mM Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS