Positive WB detected in:Hela whole cell lysate,K562 whole cell lysate,MCF-7 whole cell lysate,PC3 whole cell lysate,Mouse heart tissue;All lanes: ATP5A1 antibody at 2.5ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 60,55,58 kDa;Observed band size: 60,58 kDa;
ATP5A1 / ATP Synthase Alpha Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C498413-50
Catalog Number: LS-C498413
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- Description
- ATP Synthase Alpha antibody LS-C498413 is an unconjugated rabbit polyclonal antibody to ATP Synthase Alpha (ATP5A1) from human. It is reactive with human and mouse. Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATP5AL2 | ATP5A1 | ATP5A | HATP1 | Mitochondrial ATP synthase | MOM2 | ORM | OMR | ATPM
- UniProt Number
- P25705
- NCBI GenBank
- NM_004046 | NP_004037.1
- SwissProt
- P25705
Target
- Target
- Human ATP5A1 / ATP Synthase Alpha
- Antigen Species
- Human
- Epitope
- Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
- Gene Name
- ATP5F1A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Human ATP synthase subunit alpha, mitochondrial protein (44-257AA)
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS