LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.
AR / Androgen Receptor Antibody (phospho-Ser213, clone 156C135.2)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Non-Human Primate |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C498-0.1
Catalog Number: LS-C498
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- Description
- Androgen Receptor antibody LS-C498 is an unconjugated mouse monoclonal antibody to Androgen Receptor (AR) (pSer213) from human. It is reactive with human and monkey. Validated for WB.
- Application
- WB
- Reactivity
- Hu, NHP
- Predicted Reactivity
- Mammal (Other)
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AR | Androgen receptor | Dihydrotestosterone receptor | DHTR | AIS | KD | NR3C4 | HUMARA | SBMA | TFM | SMAX1 | HYSP1
- Recommended Dilution: WB
- 1 - 4 µg/ml
- UniProt Number
- P10275
- NCBI GenBank
- NM_000044 | NP_000035.2
- SwissProt
- P10275
Target
- Target
- Human AR / Androgen Receptor
- Antigen Species
- Human
- Epitope
- In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kD band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
- Gene Name
- AR
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 156C135.2
- Isotype
- IgG
Immunogen
- Immunogen
- This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence. Percent identity by BLAST analysis: Human, Chimpanzee, Baboon, Monkey, Marmoset (100%); Gorilla, Bat, Panda (93%); Dog, Bovine, Cat, Rabbit, Horse, Pig, Guinea pig (87%); Mouse, Rat, Hamster (80%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.05% Sodium Azide, 0.2% Gelatin
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: 4°C; Long term: -70°C.
- Recommended Storage Buffer
- PBS