Immunofluorescence Microscopy of Rabbit Anti-beta Amyloid pyro E3 antibody. Tissue: human brain section. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: beta Amyloid pyro E3 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: beta Amyloid pyro E3 is nuclear and cytoplasmic. Staining: Top: Beta Amyloid pyro E3 as as green fluorescent signal, Beta Amyloid 3 as yellow signal; and Bottom: Beta Amyloid 3 as red signal with co-incubation of Beta Amyloid pyro E3 peptide.
APP / Beta Amyloid Precursor Antibody (Pyro-Glu3)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine, Human, Non-Human Primate, Sheep |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C209352-50
Catalog Number: LS-C209352
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- Description
- Beta Amyloid Precursor antibody LS-C209352 is an unconjugated rabbit polyclonal antibody to Beta Amyloid Precursor (APP) (Pyro-Glu3) from human. It is reactive with human, bovine, primate and other species. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Bov, Hu, NHP, Sh
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ABPP | A4 | ABETA | AD1 | Alzheimer disease | Amyloid beta | APP | Beta-amyloid peptide | APPI | Beta amyloid | CVAP | AAA | PN2 | PreA4 | Amyloid beta A4 protein | Peptidase nexin-II | CTFgamma | PN-II | Protease nexin-II
- Recommended Dilution: ELISA
- 1:20000 - 1:60000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:100 - 1:500
- UniProt Number
- P05067
- NCBI GenBank
- NM_000484 | NP_000475.1
- SwissProt
- P05067
Target
- Target
- Human APP / Beta Amyloid Precursor
- Antigen Species
- Human
- Epitope
- This antibody contains no reactivity towards the 1-42 ABeta peptide. A BLAST analysis was used to suggest cross-reactivity with Human, Primate, Bovine and Sheep based on 100% sequence homology. It is not reactive with Rodentia. Cross-reactivity with beta Amyloid pyro Glu3 from other sources has not been determined.
- Gene Name
- APP
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Beta Amyloid [Pyro Glu3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminus 3-pyro E start point of human beta Amyloid.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl