Immunofluorescence microscopy of rabbit Anti-Beta Amyloid antibody using HeLa cells fixed with MeOH. Anti-Beta Amyloid Antibody was used at 1 µg/mL, O/N at 4°C. Secondary antibody: Anti-RABBIT IgG DyLight 488 Conjugated Preadsorbed at 2 ug/ml for 1 h at RT. Localization: APP is a cell surface protein that rapidly becomes internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles. Colocalizes with other proteins in a vesicular pattern in cytoplasm and perinuclear regions. Staining: Amyloid beta as green fluorescent signal with DAPI (blue) nuclear counterstain.
APP / Beta Amyloid Precursor Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745284-25
Catalog Number: LS-C745284
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- Description
- Beta Amyloid Precursor antibody LS-C745284 is an unconjugated rabbit polyclonal antibody to Beta Amyloid Precursor (APP) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ABPP | A4 | ABETA | AD1 | Alzheimer disease | Amyloid beta | APP | Beta-amyloid peptide | APPI | Beta amyloid | CVAP | AAA | PN2 | PreA4 | Amyloid beta A4 protein | Peptidase nexin-II | CTFgamma | PN-II | Protease nexin-II
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: IF/ICC
- 1:50 - 1:200
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- P05067
- NCBI GenBank
- NM_000484 | NP_000475.1
- SwissProt
- P05067
Target
- Target
- Human APP / Beta Amyloid Precursor
- Antigen Species
- Human
- Epitope
- Cross reactivity with beta amyloid from other species is likely but has not been determined.
- Gene Name
- APP
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the amino terminus (aa 1-14) of human beta amyloid conjugated to KLH using maleimide.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.93 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl