Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG5 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG5 Antibody (D) Composite.
APG5 / ATG5 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$451.00each
Catalog Number: LS-C775321-100
Catalog Number: LS-C775321
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- Description
- ATG5 antibody LS-C775321 is an unconjugated rabbit polyclonal antibody to human ATG5 (APG5). Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- APG5 | APG5-LIKE | APG5L | Apoptosis specific protein | Apoptosis-specific protein | ATG5 | Autophagy related 5 | HAPG5 | ASP | Autophagy protein 5
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9H1Y0
- NCBI GenBank
- NM_004849 | NP_004840.1
- SwissProt
- Q9H1Y0
Target
- Target
- Human APG5 / ATG5
- Antigen Species
- Human
- Epitope
- Predicted molecular weight at ~32.4 kDa. Observed molecular weights at ~48-60 kDa based on ATG12-ATG5 heterodimer.
- Gene Name
- ATG5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the N-Terminus of human ATG5
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS