Positive WB detected in:Mouse brain tissue;All lanes:ATG4B antibody at 3.4?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 45,53,32,38,43 KDa;Observed band size: 45 KDa;
APG4B / ATG4B Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C498425-50
Catalog Number: LS-C498425
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- ATG4B antibody LS-C498425 is an unconjugated rabbit polyclonal antibody to ATG4B (APG4B) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- APG4 autophagy 4 homolog B | AUTL1 | Autophagin-1 | ATG4B | Cysteine protease ATG4B | HAPG4B | APG4B | KIAA0943
- UniProt Number
- Q9Y4P1
- NCBI GenBank
- NM_013325 | NP_037457.3
- SwissProt
- Q9Y4P1
Target
- Target
- Human APG4B / ATG4B
- Antigen Species
- Human
- Epitope
- Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
- Gene Name
- ATG4B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Human Cysteine protease ATG4B protein (350-400AA)
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS