Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~15kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~15kDa. Other Band(s): ATG12-ATG5 Complex.
APG12 / ATG12 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$451.00each
Catalog Number: LS-C775281-100
Catalog Number: LS-C775281
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- Description
- ATG12 antibody LS-C775281 is an unconjugated rabbit polyclonal antibody to human ATG12 (APG12). Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Autophagy-related protein 12 | APG12L | Autophagy related 12 | ATG12 | FBR93 | APG12 | APG12-like | Ubiquitin-like protein ATG12 | HAPG12
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O94817
- NCBI GenBank
- NM_004707 | NP_004698.3
- SwissProt
- O94817
Target
- Target
- Human APG12 / ATG12
- Antigen Species
- Human
- Epitope
- Predicted molecular weight at ~15.1 kDa. Observed molecular weights at ~48-55 kDa based on ATG12-ATG5 heterodimer.
- Gene Name
- ATG12
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the C-Terminus of human ATG12
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS