APEX1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-APEX1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-APEX1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa.
APEX1 / APE1 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C817766-100
Catalog Number: LS-C817766
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- APE1 antibody LS-C817766 is an unconjugated rabbit polyclonal antibody to human APE1 (APEX1). Validated for ELISA, ICC, IF, IHC, IP and WB.
- Application
- ELISA, ICC, IF, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AP endonuclease class I | AP lyase | APE | APE-1 | APEN | APEX1 | APE1 | APEX nuclease | Redox factor-1 | AP endonuclease 1 | APEX | APX | Protein REF-1 | REF-1 | REF1
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P27695
- NCBI GenBank
- NM_001641 | NP_001632.2
- SwissProt
- P27695
Target
- Target
- Human APEX1 / APE1
- Antigen Species
- Human
- Epitope
- Human APE1/APEX1
- Gene Name
- APEX1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human APEX1/APE1/Ref-1 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS