Immunofluorescence staining of Apex1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-MOUSE Apex1 polyclonal antibody (dilution ratio: 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
APEX1 / APE1 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815739-100
Catalog Number: LS-C815739
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- Description
- APE1 antibody LS-C815739 is an unconjugated rabbit polyclonal antibody to mouse APE1 (APEX1). Validated for ICC, IF, IHC, IP and WB.
- Application
- ICC, IF, IHC-P, IP, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AP endonuclease class I | AP lyase | APE | APE-1 | APEN | APEX1 | APE1 | APEX nuclease | Redox factor-1 | AP endonuclease 1 | APEX | APX | Protein REF-1 | REF-1 | REF1
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:200 - 1:1000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P27695
- NCBI GenBank
- NM_001641 | NP_001632.2
- SwissProt
- P27695
Target
- Target
- Mouse APEX1 / APE1
- Antigen Species
- Mouse
- Epitope
- Mouse APE1/APEX1
- Gene Name
- APEX1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the N-Terminus of Mouse APEX1/APE1/Ref-1
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS