IHC analysis of APE1 using anti-APE1 antibody. APE1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-APE1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APEX1 / APE1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C756627-100
Catalog Number: LS-C756627
For bulk orders or custom formulations:
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- Description
- APE1 antibody LS-C756627 is an unconjugated mouse monoclonal antibody to human APE1 (APEX1). Validated for Flow, ICC, IHC and WB.
- Application
- FC, ICC, IHC-F, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AP endonuclease class I | AP lyase | APE | APE-1 | APEN | APEX1 | APE1 | APEX nuclease | Redox factor-1 | AP endonuclease 1 | APEX | APX | Protein REF-1 | REF-1 | REF1
- Recommended Dilution: FC
- 1 - 3 µg/10E6 cells
- Recommended Dilution: IF/ICC
- 0.5 - 1 µg/ml
- Recommended Dilution: IHC-F
- 0.5 - 1 µg/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 0.1 - 0.5 µg/ml
- UniProt Number
- P27695
- NCBI GenBank
- NM_001641 | NP_001632.2
- SwissProt
- P27695
Target
- Target
- Human APEX1 / APE1
- Antigen Species
- Human
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- APEX1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Isotype
- IgG2b
- Origin Species
- Human
Immunogen
- Immunogen
- E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat APE1, respectively.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
- Recommended Storage Buffer
- NaCl