Western blot analysis using anti-Apaf-1 (mouse/rat), MAb (13F11) on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1 KO mice.
APAF1 / APAF-1 Antibody (aa1-97, clone 13F11)
Primary AntibodyLSBio Guarantee
| Antibody: | Rat Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunoprecipitation, Western Blot |
| Reactivity: | Mouse, Rat |
| Format: | Liquid |
$430.00each
Catalog Number: LS-C344794-100
Catalog Number: LS-C344794
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- Description
- APAF-1 antibody LS-C344794 is an unconjugated rat monoclonal antibody to APAF-1 (APAF1) (aa1-97) from mouse. It is reactive with mouse and rat. Validated for ELISA, ICC, IP and WB.
- Application
- ELISA, ICC, IP, WB
- Reactivity
- Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- APAF-1 | APAF1 | CED4 | KIAA0413
- Recommended Dilution: IP
- 1:1000 - 1:2000
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- O14727
- NCBI GenBank
- NM_013229 | NP_037361.1
- SwissProt
- O14727
Target
- Target
- Mouse APAF1 / APAF-1
- Antigen Species
- Mouse
- Epitope
- Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1.
- Gene Name
- APAF1
Antibody
- Host Species
- Rat
- Clonality
- Monoclonal
- Clone
- 13F11
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Recombinant mouse Apaf-1 (aa 1-97) containing the N-Terminus CARD domain.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.02% Sodium Azide
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS