Western blot analysis of extracts of 293T and NIH/3T3 cells, using Phospho-AP2M1-T156 antibody at 1:1000 dilution. 293T cells were treated by Calyculin A for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Hydrogen Peroxide (2nM) for 15 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 30s.
AP50 / AP2M1 Antibody (phospho-Thr156)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$293.00each
Catalog Number: LS-C750630-20
Catalog Number: LS-C750630
For bulk orders or custom formulations:
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- Description
- AP2M1 antibody LS-C750630 is an unconjugated rabbit polyclonal antibody to AP2M1 (AP50) (pThr156) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Adaptin-mu2 | AP-2 complex subunit mu | AP-2 mu 2 chain | AP-2 mu chain | AP50 | CLAPM1 | Mu2 | KIAA0109 | AP2M1 | HA2 50 kDa subunit
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q96CW1
- NCBI GenBank
- NM_004068 | NP_004059.2
- SwissProt
- Q96CW1
Target
- Target
- Human AP50 / AP2M1
- Antigen Species
- Human
- Gene Name
- AP2M1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS