AP1B1 was immunoprecipitated using: Lane A: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate. Lane C:0.5 mg Hela Whole Cell Lysate. 1 uL anti-AP1B1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-AP1B1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 105 kDa. Observed band size: 112 kDa.
AP1B1 / BAM22 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815526-100
Catalog Number: LS-C815526
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- Description
- BAM22 antibody LS-C815526 is an unconjugated rabbit polyclonal antibody to human BAM22 (AP1B1). Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ADTB1 | AP-1 complex subunit beta-1 | AP105A | AP1B1 | BAM22 | Beta1-adaptin | CLAPB2 | Beta-1-adaptin | Beta-adaptin 1 | Beta-prime-adaptin
- Recommended Dilution: WB
- 1:500 - 1:1000
- NCBI GenBank
- NM_001127
Target
- Target
- Human AP1B1 / BAM22
- Antigen Species
- Human
- Epitope
- Human Clathrin/BAM22
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A synthetic peptide corresponding to the N-Terminus of Human BAM22/AP1B1
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS