Western blot detection of TEM8 in TEM8 transfected cell lysate using antibody at 1 ug/ml.
ANTXR1 / TEM8 Antibody (aa481-497, clone 200C1339(SB20))
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Dog, Human, Non-Human Primate, Rabbit |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C596-0.1
Catalog Number: LS-C596
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- Description
- TEM8 antibody LS-C596 is an unconjugated mouse monoclonal antibody to TEM8 (ANTXR1) (aa481-497) from human. It is reactive with human, dog, monkey and other species. Validated for WB.
- Application
- IHC, WB
- Reactivity
- Can, Hu, NHP, Rb
- Predicted Reactivity
- Mammal (Other), Mouse, Pig, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- ANTXR1 | Tumor endothelial marker 8 | Anthrax toxin receptor 1 | TEM8
- Recommended Dilution: WB
- 1 - 3 µg/ml
- UniProt Number
- Q9H6X2
- NCBI GenBank
- NM_018153 | NP_060623.2
- SwissProt
- Q9H6X2
Target
- Target
- Human ANTXR1 / TEM8
- Antigen Species
- Human
- Epitope
- TEM8 is observed at ~80 kD in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kD and 85 kD double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kD band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail. Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate.
- Gene Name
- ANTXR1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 200C1339(SB20)
- Isotype
- IgG1
Immunogen
- Immunogen
- This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Panda, Rabbit (100%); Mouse, Rat, Bat, Elephant, Pig (94%); Horse, Opossum (88%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.05% Sodium Azide, 0.2% Gelatin
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: 4°C; Long term: -70°C.
- Recommended Storage Buffer
- PBS