Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin R Monoclonal Antibody, Clone S388A-10. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin R Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin R Antibody. (D) Composite.
ANK1 / Ankyrin Antibody (clone S388A-10, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$485.00each
Catalog Number: LS-C775923-100
Catalog Number: LS-C775923
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- Description
- Ankyrin antibody LS-C775923 is a biotin-conjugated mouse monoclonal antibody to Ankyrin (ANK1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- ANK-1 | Ankyrin-1 | Ankyrin-R | Erythrocyte ankyrin | ANK | ANK1 | Ankyrin | Ankyrin 1, erythrocytic | SPH2 | SPH1
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P16157
- NCBI GenBank
- NM_020476 | NP_065209.2
- SwissProt
- P16157
Target
- Target
- Human ANK1 / Ankyrin
- Antigen Species
- Human
- Epitope
- Detects ~200 kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.
- Gene Name
- ANK1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- S388A-10
- Isotype
- IgG2b
- Origin Species
- Human
Immunogen
- Immunogen
- Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS