Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using AML1 (Phospho-Ser435) antibody.
AML1 / RUNX1 Antibody (phospho-Ser435)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C825533-100
Catalog Number: LS-C825533
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- Description
- RUNX1 antibody LS-C825533 is an unconjugated rabbit polyclonal antibody to RUNX1 (AML1) (pSer435) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AMLCR1 | Aml1 oncogene | AML1 | AML1-EVI-1 | CBF-alpha-2 | CBFA2 | Acute myeloid leukemia 1 | EVI-1 | PEBP2A2 | PEA2-alpha B | AML1-EVI-1 fusion protein | Oncogene AML-1 | PEBP2-alpha B | PEBP2aB | RUNX1
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q01196
- NCBI GenBank
- NM_001754 | NP_001745.2
- SwissProt
- Q01196
Target
- Target
- Human AML1 / RUNX1
- Antigen Species
- Human
- Epitope
- AML1 (Phospho-Ser435) antibody detects endogenous levels of AML1 only when phosphorylated at serine 435. (Human: Ser435; Mouse: Ser435; Rat: Ser435)
- Gene Name
- RUNX1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human AML1 around the phosphorylation site of serine 435 (S-N-SP-P-T).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl