HeLa whole cell lysate probed with Rat anti-Tubulin Alpha:HRP This image was taken for the unconjugated form of this product. Other forms have not been tested.

Alpha Tubulin Antibody (clone YL1/2, HRP)

Primary AntibodyLSBio Guarantee

Antibody:	Rat Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Frozen, Western Blot
Reactivity:	Fungus / Yeast / Mold
Format:	Liquid

$350.00each

Catalog Number: LS-C188755-0.1

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Alpha Tubulin antibody LS-C188755 is an HRP-conjugated rat monoclonal antibody to yeast Alpha Tubulin. Validated for ELISA, IHC and WB.
Application: ELISA, IHC-F, WB
Reactivity: Fung
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: WB: 1:100 - 1:1000

Target

Target: Yeast Alpha Tubulin
Antigen Species: Fungus / Yeast / Mold
Epitope: Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.

Antibody

Host Species: Rat
Clonality: Monoclonal
Clone: YL1/2
Isotype: IgG2a

Immunogen

Immunogen: Yeast tubulin.

Format

Conjugate/Label: Horseradish Peroxidase
Format: Liquid
Formulation: PBS, 0.01% Thimerosal
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: Store at 4°C. Do not freeze. Store undiluted. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer: PBS