Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser523 5-LO. The immunolabeling is completely eliminated by treatment with l-Ptase.
ALOX5 / 5-LOX Antibody (phospho-Ser523)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Rat |
| Format: | Liquid |
$479.00each
Catalog Number: LS-C178-100
Catalog Number: LS-C178
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- Description
- 5-LOX antibody LS-C178 is an unconjugated rabbit polyclonal antibody to 5-LOX (ALOX5) (pSer523) from human. It is reactive with human and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 5-lipoxygenase | 5LPG | 5-LO | 5-LOX | Arachidonate 5-lipoxygenase | LOG5 | LOX-5 | Leukotriene A4 synthase | ALOX5 | p-Ser523 5-Lipoxgenase
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P09917
- NCBI GenBank
- NM_000698 | NP_000689.1
- SwissProt
- P09917
Target
- Target
- Human ALOX5 / 5-LOX
- Antigen Species
- Human
- Epitope
- Specific for the ~80k 5-LO phosphorylated at Ser523 in Western blots
- Gene Name
- ALOX5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A phosphopeptide corresponding to amino acid residues surrounding the phosphoSer523 of human 5-lipoxygenase.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% Glycerol
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 10 mM HEPES|150 mM NaCl