Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of Anti-AKT (RABBIT) Antibody. Lane 1: SuperSignal MW markers. Lane 2: rAKT1. Lane 3: rAKT2. Lane 4: rAKT3. Load: 50 ng per lane. Primary antibody: Anti- AKT (RABBIT) Antibody at 1:1,000 for 2 hours at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:20,000 for 1 hour at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070), 1/2 hour at RT. Predicted/Observed size: 56kDa, 56kDa for AKT1, 2, and 3.
AKT1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745008-100
Catalog Number: LS-C745008
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- AKT1 antibody LS-C745008 is an unconjugated rabbit polyclonal antibody to AKT1 from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
- Application
- ELISA, FC, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Predicted Reactivity
- Chicken
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
- Recommended Dilution: IF/ICC
- 1:500 - 1:2000
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- P31749
- NCBI GenBank
- NM_005163 | NP_005154.2
- SwissProt
- P31749
Target
- Target
- Human AKT1
- Antigen Species
- Human
- Epitope
- Reacts with the AKT from human tissues. Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
- Gene Name
- AKT1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.5 M NaCl
