Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15', using AKT1/3 (Phospho-Tyr437/434) Antibody.
AKT1 + AKT3 Antibody (phospho-Tyr473)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$341.00each
Catalog Number: LS-C291543-50
Catalog Number: LS-C291543
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- Description
- AKT1 + AKT3 antibody LS-C291543 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT3 (pTyr473) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, Peptide-ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
Target
- Target
- Human AKT1 + AKT3
- Antigen Species
- Human
- Epitope
- AKT1/3 (Phospho-Tyr437/434) Antibody detects endogenous levels of AKT1/AKT3 only when phosphorylated at Y473.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic phospho-peptide derived from human AKT1/3 around the phosphorylation site of Tyr473.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS|150 mM NaCl