Akt Substrate Antibody (phospho-Ser/Thr)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Format: | Liquid |
$703.00each
Catalog Number: LS-C63885-100
Catalog Number: LS-C63885
For bulk orders or custom formulations:
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- Description
- Akt Substrate antibody LS-C63885 is an unconjugated rabbit polyclonal antibody to Akt Substrate (pSer/Thr). Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IHC-P, IP, WB
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: IP
- 1:20
- Recommended Dilution: WB
- 1:1000
Target
- Target
- Akt Substrate
- Epitope
- Preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA the antibody recognizes a wide range of phosphorylated Akt substrate peptides and by 2D gel Western blot analysis it recognizes a large number of presumptive Akt substrate proteins.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic phospho- Akt substrate peptides.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 50% Glycerol, 0.1mg/ml BSA
- Concentration
- 0.083 mg/mL
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 10 mM HEPES|150 mM NaCl
