Immunofluorescence staining of SCYE1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SCYE1 polyclonal antibody (dilution ratio 1:500) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
AIMP1 / EMAP II Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C817983-100
Catalog Number: LS-C817983
For bulk orders or custom formulations:
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- Description
- EMAP II antibody LS-C817983 is an unconjugated rabbit polyclonal antibody to human EMAP II (AIMP1). Validated for ELISA, ICC, IF, IHC, IP and WB.
- Application
- ELISA, ICC, IF, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AIMP1 | EMAP II | EMAPII | EMAP-2 | HLD3 | p43 | EMAP2 | SCYE1
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:200 - 1:1000|1:200 - 1:000
- Recommended Dilution: IHC-P
- 1:1000 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q12904
- NCBI GenBank
- NM_004757 | NP_004748.2
- SwissProt
- Q12904
Target
- Target
- Human AIMP1 / EMAP II
- Antigen Species
- Human
- Epitope
- Human EMAP-II/AIMP1
- Gene Name
- AIMP1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human EMAP-II/AIMP1 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS