Western blot analysis of extracts of various cell lines, using AFG3L2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

AFG3L2 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C750363-20

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Overview

Description: AFG3L2 antibody LS-C750363 is an unconjugated rabbit polyclonal antibody to AFG3L2 from human. It is reactive with human and mouse. Validated for WB.
Application: WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: AFG3-like protein 2 | AFG3L2 | SPAX5 | Spinocerebellar ataxia 28 | Paraplegin-like protein | ATPase family gene 3, yeast | SCA28
Recommended Dilution: WB: 1:200 - 1:2000
UniProt Number: Q9Y4W6
NCBI GenBank: NM_006796 | NP_006787.2
SwissProt: Q9Y4W6

Target

Target: Human AFG3L2
Antigen Species: Human
Gene Name: AFG3L2

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 538-797 of human AFG3L2 (NP_006787.2). DSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)