Flow Cytometry analysis of U20S cells using anti-ADA antibody. Overlay histogram showing U20S cells stained with anti-ADA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ADA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ADA / Adenosine Deaminase Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C756634-100
Catalog Number: LS-C756634
For bulk orders or custom formulations:
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- Description
- Adenosine Deaminase antibody LS-C756634 is an unconjugated mouse monoclonal antibody to human Adenosine Deaminase (ADA). Validated for Flow, IHC and WB.
- Application
- FC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Adenosine deaminase | ADA1 | ADA | Adenosine aminohydrolase
- Recommended Dilution: FC
- 1 - 3 µg/10E6 cells
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 0.1 - 0.5 µg/ml
- UniProt Number
- P00813
- NCBI GenBank
- NM_000022 | NP_000013.2
- SwissProt
- P00813
Target
- Target
- Human ADA / Adenosine Deaminase
- Antigen Species
- Human
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- ADA
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Isotype
- IgG2b
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
- Recommended Storage Buffer
- NaCl