Detection of Human Actin by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, rabbit anti-Actin antibody used at 0.07 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ACTB / Beta Actin Antibody (N-Terminus, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Lyophilized |
$466.00each
Catalog Number: LS-C287380-100
Catalog Number: LS-C287380
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- Description
- Beta Actin antibody LS-C287380 is a biotin-conjugated rabbit polyclonal antibody to Beta Actin (ACTB) (N-Terminus) from human. It is reactive with human and mouse. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Bird / Avian (Other), Bovine, Chicken, Dog, Guinea Pig, Hamster, Horse, Mammal (Other), Pig, Rabbit, Rat, Sheep, Xenopus
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Actin, cytoplasmic 1 | ACTB | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
- Recommended Dilution: WB
- 1:5000 - 1:15000
- UniProt Number
- P60709
- NCBI GenBank
- NM_001101 | NP_001092.1
- SwissProt
- P60709
Target
- Target
- Human ACTB / Beta Actin
- Antigen Species
- Human
- Epitope
- The epitope recognized by A300-491B maps to the N-terminus of human Beta-actin using the numbering given in Swiss-Prot entry P60709 (GeneID 60).
- Gene Name
- ACTB
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 60 corresponding to amino acids near the N-Terminus of UniprotID: P60709
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from PBS, 20% Trehalose, 0.2% BSA, and 0.09% Sodium Azide
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to 6 months after reconstitution.
- Recommended Storage Buffer
- PBS