Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 44 kDa.
ACTB / Beta Actin Antibody (clone 10, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$172.00each
Catalog Number: LS-C820800-20
Catalog Number: LS-C820800
For bulk orders or custom formulations:
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- Description
- Beta Actin antibody LS-C820800 is an unconjugated mouse monoclonal antibody to human Beta Actin (ACTB). Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Actin, cytoplasmic 1 | ACTB | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
- Recommended Dilution: WB
- 1:2500 - 1:200000
- UniProt Number
- P60709
- NCBI GenBank
- NM_001101 | NP_001092.1
- SwissProt
- P60709
Target
- Target
- Human ACTB / Beta Actin
- Antigen Species
- Human
- Epitope
- Human Beta-Actin
- Gene Name
- ACTB
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 10
- Isotype
- IgG2a
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the N-Terminus of Human beta Actin / ACTB
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 20 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS