Dot Blot results of rabbit Anti-Acid Phosphatase Biotin Conjugated. Antigen: Acid Phosphatase. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Acid Phosphatase Biotin 10µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.
ACP1 / Acid Phosphatase Antibody (Liquid, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Plant |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745123-25
Catalog Number: LS-C745123
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- Description
- Acid Phosphatase antibody LS-C745123 is a biotin-conjugated rabbit polyclonal antibody to potato Acid Phosphatase (ACP1). Validated for DB, ELISA and WB.
- Application
- DB, ELISA, WB
- Reactivity
- Plnt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Acid Phosphatase | Acid phosphatase 1, soluble | ACP1 | Acid phosphatase 1 soluble | Adipocyte acid phosphatase | LMW-PTP | HAAP | Red cell acid phosphatase 1 | LMW-PTPase | Protein tyrosine phosphatase
- UniProt Number
- P24666
- NCBI GenBank
- NM_004300 | NP_004291.1
- SwissProt
- P24666
Target
- Target
- Potato ACP1 / Acid Phosphatase
- Antigen Species
- Plant
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato]. Cross reactivity against Acid Phosphatase from other sources is unknown.
- Gene Name
- ACP1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Acid Phosphatase [Potato].
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl