Staining K562 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ABL1 / c-ABL Antibody (phospho-Tyr393,pTyr412)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Non-Human Primate |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C800899-100
Catalog Number: LS-C800899
For bulk orders or custom formulations:
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- Description
- C-ABL antibody LS-C800899 is an unconjugated rabbit polyclonal antibody to c-ABL (ABL1) (pTyr393,pTyr412) from human. It is reactive with human, mouse and monkey. Validated for IF, IHC, Peptide-ELISA and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ABL1 | Bcr/c-abl oncogene protein | Bcr-Abl | Bcr/abl | C-ABL | ABL | JTK7 | p150 | Tyrosine-protein kinase ABL1 | Proto-oncogene c-Abl | V-abl
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P00519
- NCBI GenBank
- NM_005157 | NP_005148.2
- SwissProt
- P00519
Target
- Target
- Human ABL1 / c-ABL
- Antigen Species
- Human
- Epitope
- Phospho-Abl (Tyr393/412) Antibody detects endogenous levels of Abl only when phosphorylated at Tyrosine 393/412.
- Gene Name
- ABL1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human Abl around the phosphorylation site of Tyrosine 393/412.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS