Western Blot of rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder Lane 1: MEF3 KO. Lane 2: MEF3 WT. Lane 3: Mouse brain. Lane 4: Rat brain. Lane 5: HCT-116 WCL. Lane 6: HeLa WCL. Lane 7: NIH/3T3 WCL. Load: 35 µg per lane. Primary antibody: Abi1 antibody at 1:1,000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.
ABI1 / SSH3BP1 Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745399-100
Catalog Number: LS-C745399
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- Description
- SSH3BP1 antibody LS-C745399 is an unconjugated rabbit polyclonal antibody to mouse SSH3BP1 (ABI1) (C-Terminus). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Ms
- Predicted Reactivity
- Human, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Abelson interactor 1 | ABI1 | Abl-interactor protein 1 long | ABI-1 | Abl-interactor 1 | Abl interactor 1 | Abl-binding protein 4 | ABLBP4 | NAP1BP | Nap1 binding protein | SSH3BP1 | Nap1-binding protein | E3B1 | Eps8-binding protein | Interactor protein AblBP4 | SSH3BP
- Recommended Dilution: ELISA
- 5 ug/ml
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- Q8IZP0
- NCBI GenBank
- NM_005470 | NP_005461.2
- SwissProt
- Q8IZP0
Target
- Target
- Mouse ABI1 / SSH3BP1
- Antigen Species
- Mouse
- Epitope
- A BLAST analysis was used to suggest cross reactivity with this protein in human and rat based on 100% homology for the immunogen sequence. Reactivity against homologues from other sources is not known.
- Gene Name
- ABI1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to near c-terminal of the mouse Abi-1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.07 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl