Goat anti Alpha-2-Macroglobulin antibody was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
A2M / Alpha-2-Macroglobulin Antibody (Liquid, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744715-25
Catalog Number: LS-C744715
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- Description
- Alpha-2-Macroglobulin antibody LS-C744715 is an HRP-conjugated goat polyclonal antibody to human Alpha-2-Macroglobulin (A2M). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- A2M | CPAMD5 | A2MD | FWP007 | Alpha-2-M | Alpha-2-macroglobulin | S863-7
- Recommended Dilution: ELISA
- 1:400 - 1:2000
- Recommended Dilution: IHC
- 1:50 - 1:250
- Recommended Dilution: WB
- 1:100 - 1:500
- UniProt Number
- P01023
- NCBI GenBank
- NM_000014 | NP_000005.2
- SwissProt
- P01023
Target
- Target
- Human A2M / Alpha-2-Macroglobulin
- Antigen Species
- Human
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a2-Macroglobulin [Human Plasma]. Cross reactivity against a2-Macroglobulin from other sources is unknown.
- Gene Name
- A2M
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A2-Macroglobulin [Human Plasma]
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl