Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

7-Ketocholesterol Antibody (clone 7E1, PerCP)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:	Pan-species / Cross-reactive
Format:	Liquid

See all All species 7-Ketocholesterol antibodies â†’

$544.00each

Catalog Number: LS-C821265-100

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Overview

Description: 7-Ketocholesterol antibody LS-C821265 is a PerCP-conjugated mouse monoclonal antibody to all species 7-Ketocholesterol. Validated for ELISA, IF and WB.
Application: ELISA, IF, WB
Reactivity: Pan
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA: 1:1000
Recommended Dilution: IF/ICC: 1:50
Recommended Dilution: WB: 1:1000