LSBio
5-Methylcytidine Antibody (clone 33D3)

5-Methylcytidine Antibody (clone 33D3)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Methylated DNA Immunoprecipitation, Western Blot
Reactivity:Pan-species / Cross-reactive
Format:Liquid
See all Broad 5-Methylcytidine antibodies →
$595.00each
Catalog Number: LS-C188235-0.1

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Overview

Description
5-Methylcytidine antibody LS-C188235 is an unconjugated mouse monoclonal antibody to broad 5-Methylcytidine. Validated for ELISA, Flow, IF, IHC, MthDnaIP and WB.
Application
ELISA, FC, IF, IHC-F, IHC-P, IP, meDIP, WB
Reactivity
Pan
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies

Target

Target
Broad 5-Methylcytidine
Antigen Species
Pan-species / Cross-reactive
Epitope
Recognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumors, DNA is frequently globally hypomethylated compared to the DNA from normal tissue. Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart cytosine. The antibody has been reported for use in Methylated DNA immunoprecipitation (MeDIP).

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
33D3
Isotype
IgG1

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.01% Thimerosal
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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