5-mC / 5-Methylcytosine Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Sheep Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Pan-species / Cross-reactive |
| Format: | Liquid |
$296.00each
Catalog Number: LS-C187857-0.1
Catalog Number: LS-C187857
For bulk orders or custom formulations:
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- Description
- 5-Methylcytosine antibody LS-C187857 is an unconjugated sheep polyclonal antibody to broad 5-Methylcytosine (5-mC). Validated for IF, IHC, IP and WB.
- Application
- IF, IHC-F, IHC-P, IP, WB
- Reactivity
- Pan
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: IHC-P
- 10 µg/ml
- Recommended Dilution: WB
- 1:1000
Target
- Target
- Broad 5-mC / 5-Methylcytosine
- Antigen Species
- Pan-species / Cross-reactive
- Epitope
- Sheep Anti 5-Methylcytosine (5-MeC) recognizes the methylated form of cytosine (C), an epigenetic modification catalyzed by DNA methyltransferases resulting in the attachment of a methyl group to carbon 5. This modification alters the structure of cytosine but not its base-pairing properties. The timing and function of cytosine methylation varies amongst species with roles in such processing events as transcription and recombination. For example, in mammals, methylation occurring during early development and emrbyogensis functions in X chromosome inactivation and gene imprinting, whereas in plants, DNA methylation occurs later and affects genomic stability through silencing of transposable elements. The deamination of 5-MeC results in the formation of thymine (T) instead of uracil. Since thymine is not recognized by DNA repair enzymes, cytosine to thymine conversions can cause transition mutations. The most frequent mutations in human genetic diseases are those involving C to T transitions occurring at CG sites. Another type of mutation involving 5-MeC involves the formation of pyrimidine dimers. Upon exposure to sunlight, pyrimidine dimers form preferentially at dipyrimidine sequences containing 5-MeC, a mutation frequently seen in human skin cancers. Sheep Anti-5-MeC is reported as suitable for use in immunocytochemistry.
Antibody
- Host Species
- Sheep
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Keyhole limpet hemocyanin (KLH) conjugated 5-methylcytosine.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS