Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

4-Hydroxy-2-hexenal Antibody (clone 6F10, APC)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Western Blot
Reactivity:	Pan-species / Cross-reactive
Format:	Liquid

See all All species 4-Hydroxy-2-hexenal antibodies â†’

$544.00each

Catalog Number: LS-C821036-100

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Overview

Description: 4-Hydroxy-2-hexenal antibody LS-C821036 is an APC-conjugated mouse monoclonal antibody to all species 4-Hydroxy-2-hexenal. Validated for ELISA, Flow, IF and WB.
Application: ELISA, FC, IF, WB
Reactivity: Pan
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA: 1:1000
Recommended Dilution: FC: 1:50
Recommended Dilution: IF/ICC: 1:50
Recommended Dilution: WB: 1:1000

Target

Target: All species 4-Hydroxy-2-hexenal
Antigen Species: Pan-species / Cross-reactive
Epitope: Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.