Flow Cytometry analysis using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.

4-HNE Antibody (clone 6F10, Atto 594)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Western Blot
Reactivity:	Pan-species / Cross-reactive
Format:	Liquid

$545.00each

Catalog Number: LS-C777291-100

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Overview

Description: 4-HNE antibody LS-C777291 is an Atto 594-conjugated mouse monoclonal antibody to all species 4-HNE. Validated for ELISA, Flow, IF and WB.
Application: ELISA, FC, IF, WB
Reactivity: Pan
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA: 1:1000
Recommended Dilution: FC: 1:50
Recommended Dilution: IF/ICC: 1:50
Recommended Dilution: WB: 1:1000

Target

Target: All species 4-HNE
Antigen Species: Pan-species / Cross-reactive
Epitope: Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.