LSBio
4-HNE Antibody (clone 12F4)

Dot blot analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 12F4. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.

4-HNE Antibody (clone 12F4)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Pan-species / Cross-reactive
Format:Liquid
See all All species 4-HNE antibodies →
$507.00each
Catalog Number: LS-C775489-100

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Overview

Description
4-HNE antibody LS-C775489 is an unconjugated mouse monoclonal antibody to all species 4-HNE. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Pan
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:1000
Recommended Dilution: WB
1:1000

Target

Target
All species 4-HNE
Antigen Species
Pan-species / Cross-reactive
Epitope
Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
12F4
Isotype
IgG1

Immunogen

Immunogen
Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Synthetic Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.