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IHC-plus™ Polyclonal Rabbit anti-Human SMO / Smoothened Antibody (1st Extracellular Domain, IHC, IF)

Ovary, Carcinoma

IHC-plus™ Polyclonal Rabbit anti-Human SMO / Smoothened Antibody (1st Extracellular Domain, IHC, IF)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general)
Reactivity:Human
Format:Liquid
See all SMO / Smoothened antibodies →
$403.00each
Catalog Number: LS-A2668-50

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Overview

Description
Smoothened antibody LS-A2668 is an unconjugated rabbit polyclonal antibody to Smoothened (SMO) (Cytoplasmic Domain) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Application
IHC
Reactivity
Hu
Predicted Reactivity
Bov, Can, Ck, Ham, Hr, Mml, Ms, NHP, Por, Rb, Rt, Xen
Intended Use
RUO
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Frizzled family member 11 | FZD11 | Gx | Protein Gx | SMOH | Smoothened | SMO | Smoothened homolog
Recommended Dilution: IHC-P
5 - 10 µg/ml
UniProt Number
Q99835
SwissProt
Q99835

Target

Target
SMO / Smoothened
Antigen Species
Hu
Specificity
1st Extracellular Domain of human, gibbon SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins
Epitope
Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Gene Name
SMO

Antibody

Host Species
Rb
Clonality
Polyclonal

Immunogen

Immunogen
Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (94%); Opossum (88%).

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, 0.1% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
Vertebrate Smoothened functions at the primary cilium. Corbit KC, Aanstad P, Singla V, Norman AR, Stainier DY and Reiter JF. Nature. 2005;437:1018-21. PubMed: 16136078

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