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| Catalog Number | Size | Price |
|---|---|---|
| LS-A2668-50 | 50 µg (1 mg/ml) | $460 |
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PathPlus™ Polyclonal Rabbit anti‑Human SMO / Smoothened Antibody (1st Extracellular Domain, IHC, IF) LS‑A2668
PathPlus™ Polyclonal Rabbit anti‑Human SMO / Smoothened Antibody (1st Extracellular Domain, IHC, IF) LS‑A2668
Antibody:
SMO / Smoothened Rabbit anti-Human Polyclonal (1st Extracellular Domain) Antibody
Application:
IHC, IHC-P, IF
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Overview
Antibody:
SMO / Smoothened Rabbit anti-Human Polyclonal (1st Extracellular Domain) Antibody
Application:
IHC, IHC-P, IF
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
SMO (Smoothened) is a Frizzled Receptor that associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal. This controls patterning and growth during vertebrate development. SMO is activated in a number of human tumors. It can function as an oncogene in basal-cell carcinomas, and is a target of various inhibition therapies. In immunohistochemistry, SMO has moderate membranous and cytoplasmic positivity in a majority of tissues throughout the body.
References: Cancers (Basel). 2016 Feb; 8(2): 22, PMID: 26891329
Target
Human SMO / Smoothened
Synonyms
SMO | Frizzled family member 11 | FZD11 | Gx | Protein Gx | SMOH | Smoothened | Smoothened homolog
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 16 amino acid peptide from 1st extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (94%); Opossum (88%).
Epitope
1st Extracellular Domain
Specificity
Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
- IHC
- IHC - Paraffin (1:400)
- Immunofluorescence
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SMO / Smoothened
Validation
Skin, Basal Cell Carcinoma
Skin, Basal Cell Carcinoma
Skin, Malignant Melanoma
Skin, Malignant Melanoma
Ovary, carcinoma
Ovary, carcinoma
Human, Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
Ovary, Carcinoma
Ovary, Carcinoma
Anti-SMO / Smoothened antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SMO / Smoothened antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Skin, Basal Cell Carcinoma
Skin, Basal Cell Carcinoma
See More About...
LSBio Ratings
PathPlus™ SMO / Smoothened Antibody (1st Extracellular Domain) for IHC, IF/Immunofluorescence LS-A2668 has an LSBio Rating of
Publications (4.4)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (5)
Vertebrate Smoothened functions at the primary cilium. Corbit KC, Aanstad P, Singla V, Norman AR, Stainier DY and Reiter JF. Nature. 2005 437:1018-21. (ICC; Mouse, Dog)
Basal cell carcinomas arise from hair follicle stem cells in Ptch1(+/-) mice. Wang GY, Wang J, Mancianti ML, Epstein EH Jr. Cancer cell. 2011 19:114-24. (IHC-P; Mouse)
Characterization of primary cilia and Hedgehog signaling during development of the human pancreas and in human pancreatic duct cancer cell lines. Nielsen SK, M[Character f8]llg[Character e5]rd K, Clement CA, Veland IR, Awan A, Yoder BK, Novak I, Christensen ST. Developmental dynamics : an official publication of the American Association of Anatomists. 2008 Aug;237:2039-52.
Ptch2 shares overlapping functions with Ptch1 in Smo regulation and limb development. Zhulyn O, Nieuwenhuis E, Liu YC, Angers S, Hui CC. Developmental biology. Jan;397:191-202.
Mesothelial mobilization in the developing lung and heart differs in timing, quantity, and pathway dependency. Timo H Lüdtke, Carsten Rudat, Jennifer Kurz, Regine Häfner, Franziska Greulich, Irina Wojahn, Nurullah Aydogdu, Tamrat M Mamo, Marc-Jens Kleppa, Mark-Oliver Trowe, Tobias Bohnenpoll, Makoto Mark Taketo, Andreas Kispert. American journal of physiology. Lung cellular and molecular physiology. 2019 May;316:L767-L783.
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Mouse, Human, Rat, Bovine
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
Date Requested: 4/26/2024
