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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B10666-200 200 µl $425 
NKX2-1 / Thyroid-Specific TF Antibody - Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung alveoli, Type 2 pneumocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
NKX2-1 / Thyroid-Specific TF Antibody - NKX2-1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NKX2-1 antibody detected the NKX2-1 protein (arrow).
NKX2-1 / Thyroid-Specific TF Antibody - NKX2-1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NKX2-1 / Thyroid-Specific TF Antibody - Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung alveoli, Type 2 pneumocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
NKX2-1 / Thyroid-Specific TF Antibody - NKX2-1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NKX2-1 antibody detected the NKX2-1 protein (arrow).
NKX2-1 / Thyroid-Specific TF Antibody - NKX2-1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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PathPlus™ Polyclonal NKX2‑1 / Thyroid‑Specific TF Antibody (aa1‑30, IHC, WB) LS‑B10666

PathPlus™ Polyclonal NKX2‑1 / Thyroid‑Specific TF Antibody (aa1‑30, IHC, WB) LS‑B10666

Note: This antibody replaces LS-C164895
Antibody:
Rabbit Polyclonal to Human NKX2-1 / Thyroid-Specific TF
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B10666-200
200 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human NKX2-1 / Thyroid-Specific TF
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NKX2-1 (also known as thyroid transcription factor TTF-1) is a nuclear protein involved in the differentiation of lung, thyroid and forebrain cells. It regulates the expression of surfactant proteins in the lung that are required for host defense and stability. TTF-1 is a useful marker for distinguishing a primary lung adenocarcinoma from metastases, particularly when combined with Napsin A in a marker panel. In normal tissues, it is expressed in Type II pneumocytes, Clara Cells, thyroid follicular epithelium and parafollicular C cells. NKX2-1 antibodies are useful for distinguishing primary pulmonary adenocarcinoma (positive staining) from metastases, pleural lung carcinomas (positive) from mesotheliomas, and also small cell carcinoma of the lung (positive) from Merkel cell carcinoma. NKX2-1 is positive in small cell lung carcinomas, adenocarcinomas, thyroid hyperplasias and carcinomas, and neoplasias. It is expected to have nuclear staining. References: Clin Sci. 2009 Jan. 116.1; 27-35, PMID: 19037882; Clin Onc. 2009 Jan. 27.2; 271-278, DOI:10.1200/JCO.2008.17.0043; AIMM 2002 10(2):97; Annals of Oncology 2006 17(11):1672; Am J Surg path 2001 25:363; Mod path 2000 13:570
Target
Human NKX2-1 / Thyroid-Specific TF
Synonyms
NKX2-1 | BCH | Benign chorea | Homeobox protein Nkx-2.1 | NK-2 | NKX2.1 | NK-2 homolog A | Thyroid transcription factor 1 | NK2 homeobox 1 | TTF-1 | BHC | Thyroid nuclear factor 1 | TITF1 | NKX2A | TEBP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E450 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-B10666. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1-30
Specificity
This NKX2-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NKX2-1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NKX2-1 / Thyroid-Specific TF
P43699 NM_003317 NP_003308.1

Validation

NKX2-1 / Thyroid-Specific TF Antibody - Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung alveoli, Type 2 pneumocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung alveoli, Type 2 pneumocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung alveoli, Type 2 pneumocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

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Requested From: United States
Date Requested: 2/23/2020