LSBio
WNT1 Antibody (Internal, clone 13F9)

Western blot using the protein-A purified anti-Wnt1 monoclonal antibody shows detection of Wnt1 protein in mouse testis lysate. The results show specific binding corresponding to the ~41 kDa Wnt1 protein. Primary antibody was used at a 1:500 dilution.

WNT1 Antibody (Internal, clone 13F9)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human WNT1 antibodies →
$311.00each
Catalog Number: LS-C744822-25

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Overview

Description
WNT1 antibody LS-C744822 is an unconjugated mouse monoclonal antibody to human WNT1 (Internal). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Predicted Reactivity
Bov, Can, Ck, Mml, Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
INT1 | Proto-oncogene Int-1 homolog | Proto-oncogene Wnt-1 | WNT1
Recommended Dilution: ELISA
1:4000 - 1:20000
Recommended Dilution: WB
1:175 - 1:250
UniProt Number
P04628
SwissProt
P04628

Target

Target
Human WNT1
Antigen Species
Hu
Epitope
This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.
Gene Name
WNT1

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
13F9
Isotype
IgG1
Light Chain
κ
Origin Species
Hu

Immunogen

Immunogen
Synthetic peptide corresponding to an internal region of human Wnt1 protein.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Prot A

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.