Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of Goat anti-Uricase Peroxidase Conjugated antibody. Lane 1: purified Uricase under reducing conditions. Lane 2: purified Uricase under reducing conditions. Lane 3: purified Uricase under non-reducing conditions. Lane 4: purified Uricase under non-reducing conditions Load: ~1.0 µg (Lane 1 and 3) and 0.25 µg (Lane 2 and 4). Primary antibody: Uricase Peroxidase Conjugated Antibody at 1:5,000 overnight at 4°C. Secondary antibody: DyLight 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 58 kDa, 58 kDa for Uricase. Other band(s): Uricase splice variants and isoforms.
Uricase Antibody (Lyophilized, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Bacteria |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C744702-100
Catalog Number: LS-C744702
For bulk orders or custom formulations:
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- Description
- Uricase antibody LS-C744702 is an HRP-conjugated goat polyclonal antibody to bacillus Uricase. Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Bac
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:1000 - 1:2500
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
Target
- Target
- Bacillus Uricase
- Antigen Species
- Bac
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species]. Cross reactivity against Uricase from other sources is unknown.
Antibody
- Host Species
- Gt
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.
Format
- Conjugate/Label
- HRP
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- IEX
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
