LSBio
Polyclonal Rabbit anti-Human TUBA1A / Tubulin Alpha 1a Antibody (Preservative Free, IHC, IF, WB)

Immunofluorescence staining of Alpha-Tubulin in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Alpha-Tubulin polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.

Polyclonal Rabbit anti-Human TUBA1A / Tubulin Alpha 1a Antibody (Preservative Free, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
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$212.00each
Catalog Number: LS-C815222-50

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Overview

Description
Tubulin Alpha 1a antibody LS-C815222 is an unconjugated rabbit polyclonal antibody to human Tubulin Alpha 1a (TUBA1A). Validated for ICC, IF, IHC, IP and WB.
Application
ICC, IF, IHC-P, IP, WB
Reactivity
Hu
Predicted Reactivity
Bovine, Dog, Hamster, Mouse, Pig, Rabbit, Rat, Rhesus Macaque
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Alpha-tubulin 3 | B-ALPHA-1 | Hum-a-tub1 | TUBA3 | Tubulin alpha-3 chain | Tubulin B-alpha-1 | TUBA1A | Tubulin, alpha 1a | Tubulin, alpha, brain-specific | Hum-a-tub2 | LIS3 | Tubulin Alpha 1a | Tubulin alpha-1A chain
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
1:500 - 1:2000
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q71U36
SwissProt
Q71U36

Target

Target
Human TUBA1A / Tubulin Alpha 1a
Antigen Species
Human
Epitope
Human TBA1A
Gene Name
TUBA1A

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthetic peptide corresponding to the C-Terminus of Human Alpha-Tubulin
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.2 µm filtered solution in PBS
Volume
50 Microliter
Preservative
No
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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