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Catalog Number Size Price
LS-C661993-100 100 µg $369 
TSH Receptor / TSHR Antibody - Western blot analysis of TSH Receptor using anti-TSH Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSH Receptor antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSH Receptor at approximately 86KD. The expected band size for TSH Receptor is at 86KD.
TSH Receptor / TSHR Antibody - Western blot - Anti-TSH Receptor Picoband Antibody
TSH Receptor / TSHR Antibody - Western blot analysis of TSH Receptor using anti-TSH Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSH Receptor antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSH Receptor at approximately 86KD. The expected band size for TSH Receptor is at 86KD.
TSH Receptor / TSHR Antibody - Western blot - Anti-TSH Receptor Picoband Antibody
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Polyclonal Rabbit anti‑Human TSH Receptor / TSHR Antibody (WB) LS‑C661993

Polyclonal Rabbit anti‑Human TSH Receptor / TSHR Antibody (WB) LS‑C661993

Antibody:
TSH Receptor / TSHR Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$369
LS-C661993-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
TSH Receptor / TSHR Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TSHR antibody LS-C661993 is an unconjugated rabbit polyclonal antibody to human TSHR (TSH Receptor). Validated for WB.
Target
Human TSH Receptor / TSHR
Synonyms
TSHR | CHNG1 | HTSHR-I | LGR3 | Thyrotrophin receptor | TSH-R | TSH receptor | Thyrotropin receptor
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human TSH Receptor (244-277 aa KGLEHLKELIARNTWTLKKLPLSLSFLHLTRADL), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.
Specificity
Expressed in the thyroid. .
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TSH Receptor / TSHR
P16473 NM_000369 NP_000360.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/12/2021