LSBio
Monoclonal Mouse anti-Human TRPC6 Antibody (clone 3F2.H10.F2, IHC, WB)

Immunohistochemistry using the anti-TRPC6 monoclonal antibody shows detection of TRPC6 in human adrenal (cortex) tissue (40X). The antibody was used a dilution to 2.5 µg/mL. The image shows strong staining with minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Monoclonal Mouse anti-Human TRPC6 Antibody (clone 3F2.H10.F2, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human TRPC6 antibodies →
$311.00each
Catalog Number: LS-C744836-25

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Overview

Description
TRPC6 antibody LS-C744836 is an unconjugated mouse monoclonal antibody to TRPC6 from human. It is reactive with human and chimpanzee. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Predicted Reactivity
Non-Human Primate
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Transient receptor protein 6 | Trrp6 | TRP-6 | TRP6 | FSGS2 | TRPC6
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC
2.5 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q9Y210
SwissProt
Q9Y210

Target

Target
Human TRPC6
Antigen Species
Human
Epitope
This antibody is specific for human TRPC6 protein. A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.
Gene Name
TRPC6

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
3F2.H10.F2
Isotype
IgG1
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.