IHC testing of FFPE human colon carcinoma with p53 antibody (clone Pab 1801). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
TP53 / p53 Antibody (clone PAb 1801, Azide-free, BSA-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C761813-100
Catalog Number: LS-C761813
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- P53 antibody LS-C761813 is an unconjugated mouse monoclonal antibody to human p53 (TP53). Validated for Flow, IF, IHC and WB.
- Application
- FC, IF, IHC-P, WB
- Reactivity
- Hu
- Nonreactive Species
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | TP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
- Recommended Dilution: FC
- 0.5 - 1 µg/10E6 cells
- Recommended Dilution: IF/ICC
- 1 - 2 µg/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 0.5 - 1 µg/ml
- UniProt Number
- P04637
- NCBI GenBank
- NM_000546 | NP_000537.3
- SwissProt
- P04637
Target
- Target
- Human TP53 / p53
- Antigen Species
- Human
- Epitope
- This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis. This antibody binds to an N-terminal portion of both the mutant and wild type p53.
- Gene Name
- TP53
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- PAb 1801
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Human p53 beta-galactosidase fusion protein was used as the immunogen for this antibody. Its epitope maps near the N-Terminus end (aa 32-79) of p53.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 1X PBS
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).
- Recommended Storage Buffer
- 1X PBS