LSBio
Polyclonal Rabbit anti-Human TP53 / p53 Antibody (Ac-Lys305, WB)

Dot Blot of rabbit Anti-p53 305KAc Antibody. Lane 1: Unmodified p53 peptide. Lane 2: 305KAc p53 peptide. Loaded: 100, 10, 1 picomoles of peptide. Primary Antibody: Left Membrane: 1.0 µg/ml of purified antibody; Middle Membrane: 0.5 µg/ml; Right Membrane: 0.25 µg/ml. Blocking: BLOTTO Blocking Buffer p/n B501-0500 for 30min at RT. Secondary Antibody: Gt-A-Rb IgG HRP p/n 611-103-122 at 1:40,000.

Polyclonal Rabbit anti-Human TP53 / p53 Antibody (Ac-Lys305, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human TP53 / p53 antibodies →
$311.00each
Catalog Number: LS-C745418-25

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Overview

Description
P53 antibody LS-C745418 is an unconjugated rabbit polyclonal antibody to human p53 (TP53) (Ac-Lys305). Validated for DB, ELISA and WB.
Application
DB, ELISA, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | TP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Recommended Dilution: ELISA
1:5000 - 1:10000
Recommended Dilution: WB
1 µg/ml
UniProt Number
P04637
NCBI GenBank
NM_000546, NP_000537.3
SwissProt
P04637

Target

Target
Human TP53 / p53
Antigen Species
Hu
Epitope
A BLAST analysis used to suggest reactivity with this protein from human based on 100% homology for the immunizing sequence.
Gene Name
TP53

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Anti-p53 K305 Ac antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 305 of human p53.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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