Dot Blot of rabbit Anti-p53 305KAc Antibody. Lane 1: Unmodified p53 peptide. Lane 2: 305KAc p53 peptide. Loaded: 100, 10, 1 picomoles of peptide. Primary Antibody: Left Membrane: 1.0 µg/ml of purified antibody; Middle Membrane: 0.5 µg/ml; Right Membrane: 0.25 µg/ml. Blocking: BLOTTO Blocking Buffer p/n B501-0500 for 30min at RT. Secondary Antibody: Gt-A-Rb IgG HRP p/n 611-103-122 at 1:40,000.
TP53 / p53 Antibody (Ac-Lys305)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745416-100
Catalog Number: LS-C745416
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- Description
- P53 antibody LS-C745416 is an unconjugated rabbit polyclonal antibody to human p53 (TP53) (Ac-Lys305). Validated for DB, ELISA and WB.
- Application
- DB, ELISA, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | TP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P04637
- NCBI GenBank
- NM_000546, NP_000537.3
- SwissProt
- P04637
Target
- Target
- Human TP53 / p53
- Antigen Species
- Hu
- Epitope
- A BLAST analysis used to suggest reactivity with this protein from human based on 100% homology for the immunizing sequence.
- Gene Name
- TP53
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-p53 K305 Ac antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 305 of human p53.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl