LSBio
Polyclonal Rabbit anti-Human TP53 / p53 Antibody (Ac-Lys292, WB)

Western Blot of Anti-p53 (ac Lys292) (RABBIT) Antibody. Lane 1: HCT-116 Whole Cell Lysate. Lane 2: A549 WCL. Lane 3: C5B46 Brain Lysate. Lane 4: MCF7 Nuclear Extract Lysate. Lane 5: A431 Nuclear Extract Lysate. Lane 6: HeLa WCL. Lane 7: HeLa Nuclear Extract Lysate. Lane 8: Normal Ms Brain Lysate. Lane 9: Molecular Weight Ladder PreStained Loaded at 10ug. Primary Antibody: Anti-p53 292kAc at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP Predicted: ~43.6 kDa.

Polyclonal Rabbit anti-Human TP53 / p53 Antibody (Ac-Lys292, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human TP53 / p53 antibodies →
$311.00each
Catalog Number: LS-C745409-25

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Overview

Description
P53 antibody LS-C745409 is an unconjugated rabbit polyclonal antibody to human p53 (TP53) (Ac-Lys292). Validated for DB, ELISA and WB.
Application
DB, ELISA, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | TP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Recommended Dilution: ELISA
1:5000 - 1:10000
Recommended Dilution: WB
1 µg/ml
UniProt Number
P04637
NCBI GenBank
NM_000546, NP_000537.3
SwissProt
P04637

Target

Target
Human TP53 / p53
Antigen Species
Hu
Epitope
A BLAST analysis used to suggest reactivity with this protein from human based on 100% homology for the immunizing sequence.
Gene Name
TP53

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Anti-p53 K292 Ac antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 292 of human p53.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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