LSBio
Polyclonal Rabbit anti-Human TNFAIP3 / A20 Antibody (IF, WB)

Positive WB detected in:RAW264.7 whole cell lysate,Hela whole cell lysate,A549 whole cell lysate;All lanes:TNFAIP3 antibody at 2.7ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 90 kDa;Observed band size: 90 kDa;

Polyclonal Rabbit anti-Human TNFAIP3 / A20 Antibody (IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:Human
Format:Liquid
See all Human TNFAIP3 / A20 antibodies →
$300.00each
Catalog Number: LS-C499948-50

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Overview

Description
A20 antibody LS-C499948 is an unconjugated rabbit polyclonal antibody to human A20 (TNFAIP3). Validated for ELISA, IF and WB.
Application
ELISA, IF, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
A20 | TNFA1P2 | TNFAIP3 | TNF alpha-induced protein 3 | OTUD7C | Zinc finger protein A20
UniProt Number
P21580
NCBI GenBank
NM_006290 | NP_006281.1
SwissProt
P21580

Target

Target
Human TNFAIP3 / A20
Antigen Species
Human
Epitope
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.
Gene Name
TNFAIP3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant Human Tumor necrosis factor alpha-induced protein 3 protein (97-183AA)
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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